Nail
Nail
TEST CODE
100524
CPT
87801, 87640, 87651, 87653, 87798, 87481, 87150, 87500, 87641
Expected
Turnaround Time
Turnaround Time
72 hours
Patient Preparation
Prior to sample collection, the nail plate and the surrounding soft tissue should be cleaned with wet
wipe or 70% ethanol to prevent environmental contamination.
Specimen Requirements
Specimen
Paronychia swab/Dry nail clippings, nail plate scrapings, nail bed scrapings, and subungual scrapings/debris (any or all of
these)
Volume
1 mL for swab
Container
Copan eswab/any sterile container for dry nail
Preparation
SWAB: Swab the infected area from margin to margin in a 10-point zigzag fashion. Use enough pressure to express fluid from within the tissue. Insert the swab all the way to the bottom of the tube that has a liquid medium. Holding the end of the swab shaft, bend it to break at the marked breakpoint. Label the tube with patient's identifiers and date/time of collection.
DRY NAIL:A sterile nail clipper should be used to clip the full thickness nail plate and a sterile curette or scalpel
blade should be used for scraping nail plate. Any small blunt instrument (used in pedicure) can be used
for collection of subungual debris.
Stability
Swab - Room Temperature, 4 days
Dry Nail - 15 days in Room Temperature
Storage Instructions
Room Temperature
Test Details
Methodology
Real Time PCR with TaqMan OpenArray™ Plate-format on QuantStudio 12K Flexinstrument (Applied Biosystems)
Rejection Criteria
Received past stability
Received in the improper container/non-sterile container
Collection Device expired
Tube unlabeled
Test Components
Test Code
Test Name
LOINC
Acinetobacter baumannii
61360-4
Acremonium strictum
Anaerococcus vaginalis
Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus
49863-4
Bacteroides fragilis, Bacteroides vulgatus (Bacteroides species)
Blastomyces dermatitidis
103167-3
Candida albicans, C. tropicalis, Candida parapsilosis,
101937-1
Candida glabrata, Candida kruseii, Candida auris
101940-5 97936-9
Citrobacter braakii, Citrobacter freundii
103169-9
Cladosporium herbarum
Clostridium perfringens
103172-3
Corynebacterium striatum
Curvularia lunata
Enterobacter aerogenes, Enterobacter cloacae (Enterobacter species)
103172-3
Enterococcus faecalis, E. faecium, (Enterococcii species)
103175-6
Epidermophyton floccosum
101771-4
Escherichia coli
103162-4
Fusarium moniliforme (also termed as F. verticillioides), Fusarium oxysporum, Fusarium solani
101783-9, 101782-1
Fusobacterium nucleatum, Fusobacterium necrophorum
93399-4, 93396-0
Klebsiella pneumoniae, oxytoca
103186-3
Malassezia furfur, restricta, sympodialis, globosa (Malassezia sp)
Microsporum gypseum
Morganella morganii
103188-9
Peptostreptococcus anaerobius, P. magnus (Finegoldia magna), P. prevotii (Anaerococcus prevotii), (Peptostreptococcus sp)
Prevotella bivia, loescheii (Prevotella sp)
Proteus mirabilis, vulgaris (Proteus sp)
103194-7 103196-2
Pseudomonas aeruginosa
61401-6
Salmonella enterica (subsp. enterica serovar Montevideo)
61370-3
Scopulariopsis brevicaulis
101784-7
Scytalidium hyalinum/Scytalidium dimidiatum,
Serratia marcescens
61402-4
Staphylococcus aureus
61404-0
Staphylococcus epidermidis, S. haemolyticus, Staphylococcus lugdunensis, saprophyticus (coagulase negative) (Staphylococcus sp)
Stenotrophomonas maltophilia
61403-2
Streptococcus agalactiae Group B strep (GBS)
48683-7
Streptococcus pyogenes (Group A strep)
101950-4
Trichophyton terrestre;Trichophyton verrucosum;Trichophyton violaceum
101770-6, 101767-2
Trichophyton interdigitale, Trichophyton mentagrophytes,Trichophyton tonsurans;Trichophyton rubrum;Trichophyton soudanense
101756-5,101757-3,101758-1,101764-9,101766-4,101754-0,101767-2,101770-6,69939-7
Trichosporon mucoides/Trichosporon dermatis (closely related), T. asahii
Aminoglycoside
62256-3
AmpC (Ampicillin Resistance)
85841-5
Carbapenemase blaOXA
86712-7
Carbapenemase blaPER
85837-3
Metallo-BetaLactamase
85501-5
Metallo-BetaLactamase blaIMP
85498-4 49617-4 73982-1
ESBL blaCTX
88250-6
ESBL blaOHIO blaTEM blaSHV blaVEB
85839-9 85838-1 85840-7
Vancomycin
62261-3
Macrolide
62258-9
Methicillin
48813-0
Polymixins
86221-9 93701-1 98066-4
Quinolone and fluoroquinolone 1
89487-3
Quinolone and fluoroquinolone 2
94060-1
Sulfonamide
92252-6
Tetracycline
62260-5
